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AQUEOUS TWO-PHASE SYSTEM OF POLYETHYLENE GLYCOL 6000 AND NACL FOR PARTITIONING BEHAVIOR OF L-CYSTINE

MOHAMAD, ABU RAIHAN (2011) AQUEOUS TWO-PHASE SYSTEM OF POLYETHYLENE GLYCOL 6000 AND NACL FOR PARTITIONING BEHAVIOR OF L-CYSTINE. Universiti Teknologi PETRONAS. (Unpublished)

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Abstract

The aim of this study is to investigate the feasibility of extracting of natural rubber protein, named hevein; represented by its amino acid, cysteine (L-cystine) by using aqueous two phase system (AlPS) of polyethylene glycol of molecular weight 6000 (PEG 6000) and sodium chloride. AlPS has been proposed as an alternative method as it preserved biological activity, nontoxic, and cost-effective. Purification and extraction by using polyethylene glycol/salt AlPS is very suitable since he vein can tolerate very well with inorganic solvent. Feasibility of AlPS of PEG 6000 with sodium chloride is studied where the phase diagram is developed. A precise composition of PEG 6000 with sodium chloride from the binodal curve is mixed to form two phases system to study the partitioning behavior of L-cystine where the concentration of each component and L-cystine on both phases will be analyzed. The partition behavior is recorded with respect to PEG 6000 and N aCl concentration. The highest percentage recovery of L-cystine is observed.

Item Type: Final Year Project
Academic Subject : Academic Department - Chemical Engineering - Process System Engineering
Subject: T Technology > TP Chemical technology
Divisions: Engineering > Chemical
Depositing User: Users 2053 not found.
Date Deposited: 06 Nov 2013 10:48
Last Modified: 25 Jan 2017 09:41
URI: http://utpedia.utp.edu.my/id/eprint/10160

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