AQUEOUS TWO-PHASE SYSTEM OF POLYETHYLENE GLYCOL 6000 AND NACL FOR PARTITIONING BEHAVIOR OF L-CYSTINE

The aim of this study is to investigate the feasibility of extracting of natural rubber
protein, named hevein; represented by its amino acid, cysteine (L-cystine) by using aqueous two
phase system (AlPS) of polyethylene glycol of molecular weight 6000 (PEG 6000) and sodium
chloride. AlPS has been proposed as an alternative method as it preserved biological activity,
nontoxic, and cost-effective. Purification and extraction by using polyethylene glycol/salt AlPS
is very suitable since he vein can tolerate very well with inorganic solvent. Feasibility of AlPS of
PEG 6000 with sodium chloride is studied where the phase diagram is developed. A precise
composition of PEG 6000 with sodium chloride from the binodal curve is mixed to form two
phases system to study the partitioning behavior of L-cystine where the concentration of each
component and L-cystine on both phases will be analyzed. The partition behavior is recorded
with respect to PEG 6000 and N aCl concentration. The highest percentage recovery of L-cystine
is observed.