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Thermodynamic Modeling for Protein Extraction in Water - PEG 8000 - Dextran 75 System using MATLAB

Zulkuraain, Jazlin Ernida (2009) Thermodynamic Modeling for Protein Extraction in Water - PEG 8000 - Dextran 75 System using MATLAB. Universiti Teknologi PETRONAS. (Unpublished)

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Abstract

This report basically discusses the research done and basic understanding of the chosen topic, which is Thermodynamic Modeling for Protein Extraction inAqueous Polymer System. The objective ofthe project is to study on extending the application of polymer thermodynamic model, namely Flory-Huggins Theory [1,2], to capture and predict the partitioning behaviour of a sample of different types of proteins in an Aqueous Two-Phase Extraction System (ATPES). ATPES is a liquid-liquid extraction technique that can be applied as a protein separation method. At equilibrium, these systems form water-rich two liquid phases which a target protein selectively partitions into one of the phases according to its ATPES' characteristics. The protein partitioning behavior can be captured using the above mentioned thermodynamic model. Examples oftwo-phase systems are either two polymers (e.g., PEG/dextran) or one polymer in high-salt concentration (e.g., PEG/salt). The scope for this project will be the protein extraction in aqueous polymer system. To observe the efficiency of ATPES to extract certain types ofproteins, a modeling approach by Ahmad [3] based on MATLAB will be used. Using MATLAB software as a helping hand, equilibrium compositions and the partition coefficient of a target protein in the system can be computed. The model will be used to analyze the variations in the partitioning trends and patterns for different types ofproteins. The results will be the partitioning ofthe respective target proteins, either to the top or to the bottom phase in the ATPES, producing partitioning coefficient ofgreater or less than the value of1respectively. In this report, the equilibrium compositions and the partition coefficient of human hemoglobin and lysozyme from chicken egg white in water - PEG 8000 - Dextran 75 system are calculated using the modeling approach by Ahmad [3]. The result obtained is then compared to empirical data [30].

Item Type: Final Year Project
Academic Subject : Academic Department - Chemical Engineering - Reactor Technology
Subject: T Technology > TP Chemical technology
Divisions: Engineering > Chemical
Depositing User: Users 2053 not found.
Date Deposited: 22 Oct 2013 11:22
Last Modified: 22 Oct 2013 11:22
URI: http://utpedia.utp.edu.my/id/eprint/9248

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