NANOFLOWER ENHANCED CAPACITIVE NONFARADAIC MILK ALLERGEN BIOSENSOR

The bovine milk allergenic protein, ‘β-lactoglobulin’, is one of the leading causes of milk allergy, especially in children. This research has developed a novel label-free non-faradaic capacitive aptasensor to detect β-lactoglobulin using laser-scribed graphene (LSG) electrode technology incorporated with hybrid nanoflower (NF). Firstly, the novel hybrid nanoflower was synthesized using 1,1′-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via effortless one-pot biomineralization technique by tuning the reaction time and concentration. Then, five individual capacitor-like triangular LSG electrodes were effortlessly fabricated on polyimide (PI) film with micron gap (MG) spacing of ̴ 30, 66, 95, 125 and 180 μm, via one-step CO2 laser irradiation and investigated towards dielectric biomodification. An optimum microgapped (~95 μm) sensor in terms of good reproducibility was selected to construct milk allergen biosensor.