Three-Dimensional (3D) Reconstruction of Microstructures via Serial Sectioning

A study to generate three-dimensional (3D) reconstruction images of microstructures by using serial sectioning method is performed in which, proper processes and procedures of serial sectioning method need to be established. Serial sectioning method is a material-removal technique, which only practical for a range of material-removal rates of the materials to be studied. The basic serial sectioning material removal methods are involving manual or semiautomated polishing, which can remove between approximately 0.05 to 1.0 micron per section of the material.